General information
Reference : UMR7592-KATWAS-002
Type of Contract : FTC Technical / Administrative
Contract Period : 12 months
Expected date of employment : 4 November 2024
Proportion of work : Full time
Remuneration : 2419 € to 2546 € before tax according to experience
Desired level of education : Niveau 6 – (Bac+3 ou 4)
Experience required : 1 to 4 years
BAP : Sciences du vivant, de la terre et de l’environnement
Emploi type : Ingénieur-e en techniques biologiques
Apply before September 24th 2024 on CNRS job portal
Missions
The candidate will be integrated into the “Mechanism of meiosis” team at the Jacques Monod Institute. He/she will have the mission of developing molecular, cellular biology and mass spectrometry techniques in mouse and Xenopus oocytes in order to enable the advancement of the team’s research themes.
Activities
- Construction of molecular biology tools.
- Harvest and culture of mouse oocytes, videomicroscopy.
- Collection and culture of Xenopus oocytes, preparation of extracts, western blot.
- Analysis of mouse oocytes after immunostaining by confocal and super-resolution microscopy. Mounting and quantification of acquisitions.
- Development of new techniques for preparing protein samples for analysis by mass spectrometry.
- Being proactive in contributing to the smooth functioning of the lab
- Develop and fine-tune new techniques for preparing protein samples for analysis by mass spectrometry.
- Takes the initiative in managing the laboratory and solving technical problems.
Skills
- Prior experience working in a laboratory (internships or CDD) is essential.
- Knowledge of classic molecular biology techniques (PCR, cloning, RNA preparation), and cellular biology (culture, microscopy).
- Knowing how to communicate in English is essential. No knowledge of French is required.
- Proactivity, rigor, perseverance, reliability and good ability to work in a team.
Work Context
The host team is interested in the mechanisms of cell division in meiosis, particularly in mouse and Xenopus oocytes. It is made up of 3 doctoral students, 1 assistant engineer, 3 researchers and one assistant professor. A postdoc and an engineer will join the team in early 2025. Bachelor’s and master’s students are hosted all year round.
The activity is carried out at the Jacques Monod Institute, which is a joint CNRS – Paris Cité University research unit, of around 250 people and 23 nationalities. The IJM is housed in a recent University building on the Paris Rive Gauche campus located in the 13th arrondissement of Paris and accessible by all means of transport (Metro 14, RER C, Tramway T3, bus 62 and 89.. .).
Additional Information
Our team is interested in female meiosis, a specialized division taking place in oocytes that generates haploid gametes suitable for fertilization. Meiosis consists of two divisions, meiosis I and then meiosis II. These two divisions halve cellular ploidy due to the separation of homologous chromosomes in meiosis I and the absence of intercalated S-phase. In addition, female meiosis is characterized by two cell cycle arrests: in prophase of meiosis I and then in metaphase of meiosis II, to await fertilization. Thus, the oocyte does not continue to divide without the paternal genome, a condition to produce viable embryos containing the correct number of chromosomes. Our research themes aim to decipher how the oocyte carries out and differentiates two functionally distinct divisions and then stops at the right time, a question at the heart of sexual reproduction and embryonic development.
The role and regulation of new meiotic players will be studied in mice and Xenopus using gain and loss of function approaches in order to understand their functional conservation in vertebrates. This project should therefore provide a better understanding of the mechanisms controlling female meiosis in vertebrates, a process at the heart of sexual reproduction and the cell cycle.