L'équipe Camadro (en collaboration avec ProteoSeine, Dumont Lab et Ladoux/Mège Lab) vient de publier un nouvel article dans Journal of Proteome Research : Extending the Range of SLIM-Labeling Applications: From Human Cell Lines in Culture to Caenorhabditis elegans Whole-Organism Labeling   Abstract The simple light isotope metabolic-labeling technique relies on the in vivo biosynthesis of amino acids from U-[12C]-labeled molecules…

L'équipe Minc vient de publier un nouvel article dans PNAS :   Size- and position-dependent cytoplasm viscoelasticity through hydrodynamic interactions with the cell surface   Abstract Many studies of cytoplasm rheology have focused on small components in the submicrometer scale. However, the cytoplasm also baths large organelles like nuclei, microtubule asters, or spindles that often take significant portions of…

L'équipe Dumont vient de publier un nouvel article dans eLife : Synergistic stabilization of microtubules by BUB-1, HCP-1 and CLS-2 controls microtubule pausing and meiotic spindle assembly   Abstract During cell division, chromosome segregation is orchestrated by a microtubule-based spindle. Interaction between spindle microtubules and kinetochores is central to the bi-orientation of chromosomes. Initially dynamic to allow…

L'équipe Romet-Lemonne/Jégou vient de publier dans Biophysical Journal : (ABSTRACT  VOLUME 122, ISSUE 3, SUPPLEMENT 1, 166A-167A,  FEBRUARY 10, 2023)   The Arp2/3 complex contributes to the regeneration of branched actin filament networks   Actin filaments are one of the indispensable components of almost all cell types. These semi-flexible filaments constitute an important part of the cytoskeleton and give…

DNA Modification Patterns Filtering and Analysis Using DNAModAnnot Hardy, A., Duharcourt, S., Defrance, M. (2023). DNA Modification Patterns Filtering and Analysis Using DNAModAnnot. In: H. Oliveira, P. (eds) Computational Epigenomics and Epitranscriptomics. Methods in Molecular Biology, vol 2624. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2962-8_7 Abstract Mapping DNA modifications at the base resolution is now possible at…

L'équipe Minc vient de publier un nouvel article dans PLOS Biology Cell wall dynamics stabilize tip growth in a filamentous fungus   Abstract Hyphal tip growth allows filamentous fungi to colonize space, reproduce, or infect. It features remarkable morphogenetic plasticity including unusually fast elongation rates, tip turning, branching, or bulging. These shape changes are all driven from the…

L'équipe Veitia vient de publier un nouvel article dans Cancer Research : The Oncogenic FOXL2 C134W Mutation Is a Key Driver of Granulosa Cell Tumors   Abstract Adult-type granulosa cell tumors (AGCT) are the most common type of malignant ovarian sex cord–stromal tumors. Most AGCTs carry the somatic variant c.402C>G (p.C134W) affecting the transcription factor FOXL2. Germline…

La plateforme Protoseine vient de publier un nouvel article dans Journal of Chromatography B : Capillary liquid chromatography coupled with mass spectrometry for analysis of nanogram protein quantities on a wide-pore superficially porous particle column in top-down proteomics   Abstract In top-down proteomics experiments, intact protein ions are subjected to gas-phase fragmentation for MS analysis without…

L'équipe Grange/Geigl a récemment publié un châpitre d'ouvrage dans Microbial Environmental Genomics (Part of the Methods in Molecular Biology book series (MIMB,volume 2605) Analysis of Ancient Microbial DNA Abstract The development of next-generation sequencing has led to a breakthrough in the analysis of ancient genomes, and the subsequent genomic analyses of ancient human skeletal remains have revolutionized…

L'équipe Ladoux/Mège publie un nouvel article dans Biomaterials   Bioengineering a miniaturized in vitro 3D myotube contraction monitoring chip to model muscular dystrophies   Abstract Quantification of skeletal muscle functional contraction is essential to assess the outcomes of therapeutic procedures for neuromuscular disorders. Muscle three-dimensional “Organ-on-chip” models usually require a substantial amount of biological material, which rarely…