Paris Postdoc Seminar – Lucas Alves Tavares

Invité par l’Institut Jacques Monod, Lucas Alves Tavares (Center for Virology Research and Department of Cell and Molecular Biology, University of São Paulo (USP) & Institut Curie, Paris) va présenter un Paris Postdoc Seminar sur le thème :

Unverealing the role of AP-1γ2 in the Endosome Maturation and Extracellular Vesicles Release

Résumé :

The adaptor protein (AP) complexes are heterotetrameric complexes that coordinate protein trafficking in the endocytic and secretory pathways. AP-1, a complex of four distinct subunits (γ, β1, μ1, and σ1), is thought to mediate protein trafficking between the trans-Golgi network (TGN) and endosomes and Lysosomes Related Organelles (LROs) though clathrin-coated vesicles (CCV). The human genome encodes two isoforms of the γ-adaptin (γ1 and γ2) subunit that form two variants of AP-1 (AP-1γ1 and AP-1γ2). Previous studies demonstrated that γ2 may form an AP-1 complex variant, but the knowledge is limited regarding the cellular roles played by this alternative AP-1 complex. We previously reported that γ2 is part of a functional AP-1 complex variant hijacked by HIV-1 Nef for targeting CD4 and MHC-I for lysosomal degradation. Moreover, we recently demonstrated that the retrograde transport of CI-MPR and ATP7B requires AP-1γ2. During my internship at Graça Raposo laboratory (Institut Curie), we are investigating the participation of AP-1γ2 in the maturation of early endosomes to late endosomes and also for the Extracellular Vesicles (EVs) secretion by using immunofluorescence, nanoparticle tracking analysis, western blot, immuno-electron microscopy analysis and conventional transmission electron microscopy analysis. Therefore, this study has the potential to contribute to the understanding of fundamental processes in the regulation of protein and membrane trafficking involved in the endosome maturation and EVs shedding.