Isabelle le Parco, ainsi que la plateforme qu’elle dirige, a contribué a la publication d’un article dans Communications biology :
Calcium wave dynamics in the embryonic mouse gut mesenchyme: impact on smooth muscle differentiation
Résumé :
Intestinal smooth muscle differentiation is a complex physico-biological process involving several different pathways. Here, we investigate the properties of Ca2+ waves in the developing intestinal mesenchyme using GCamp6f expressing mouse embryos and investigate their relationship with smooth muscle differentiation. We find that Ca2+ waves are absent in the pre-differentiation mesenchyme and start propagating immediately following α-SMA expression. Ca2+ waves are abrogated by CaV1.2 and gap-junction blockers, but are independent of the Rho pathway. The myosine light-chain kinase inhibitor ML-7 strongly disorganized or abolished Ca2+ waves, showing that perturbation of the contractile machinery at the myosine level also affected the upstream Ca2+ handling chain. Inhibiting Ca2+ waves and contractility with CaV1.2 blockers did not perturb circular smooth muscle differentiation at early stages. At later stages, CaV1.2 blockers abolished intestinal elongation and differentiation of the longitudinal smooth muscle, leading instead to the emergence of KIT-expressing interstitial cells of Cajal at the gut periphery. CaV1.2 blockers also drove apoptosis of already differentiated, CaV1.2-expressing smooth muscle and enteric neural cells. We provide fundamental new data on Ca2+ waves in the developing murine gut and their relation to myogenesis in this organ.
Chevalier NR, Zig L, Gomis A, Amedzrovi Agbesi RJ, El Merhie A, Pontoizeau L, Le Parco I, Rouach N, Arnoux I, de Santa Barbara P, Faure S. Calcium wave dynamics in the embryonic mouse gut mesenchyme: impact on smooth muscle differentiation. Commun Biol. 2024 Oct 7;7(1):1277. doi: 10.1038/s42003-024-06976-y. PMID: 39375515; PMCID: PMC11458798.