Spinning Disk W1 NIKON

User Manual

Capabilities

• High-speed multidimensional imaging with optical sectioning
• Spinning disk modality produces high SNR images with minimal photobleaching
• Inverted microscope
• High sensitivity sCMOS cameras
• Laser lines for all classes of fluorescent proteins

• Environmental control: temperature, CO2, humidity
• IR reflection based autofocus

• BF and Nomarski contrast (DIC)

Detailed Specifications

Microscope:

• ECLIPSE Ti2-E Fully motorized Inverted stand (Nikon)
• TI2-S-SE-E Motorized stage with linear encoding
• 500 micron piezo for rapid z-control
• Perfect Focus System (PFS)
• D-LEDI (Nikon), 4 LED : 385 nm, 475 nm, 550 nm et 621 nm for widefield illumination
• Three fluorescence cubes filters for widefield:
  DAPI Ex 390/18 nm, dichroic 416 nm, Em 460/60 nm
  GFP Ex 475/35 nm, dichroic 499 nm, Em 530/43 nm
  TXRed Ex 559/34 nm, dichroic 585 nm, Em 639/69 nm

Scanhead:

• Yokogawa CSU-W1 4000 rpm
• dichroics – (405/488/561/640) (488/561) (488)

Lasers:
405 nm, 488 nm, 561 nm, 638 nm

Emission filters:
425-475; 500-530; 500-550; 570-620; 655LP; Quad

Objectives:

• PLAN FLUOR 4x ON:0.13 DT: 17.1mm (MRH00045)
• CFI Plan APO Lambda D 10X ON:0.45 DT:4mm (MRD70170)
• CFI Plan APO Lambda D 20X ON:0.8 DT: 0.8 (MRD70270)
• PLAN APO LBDA S 40xC SIL ON:1.25 DT:0.3 (MRD73400)
• APO LBDA S 40xWI ON:1.15 DT:0.61-0.59 mm (MRD77410)
• CFI Plan APO Lambda D 60X Oil ON:1.42 DT:0.15 (MRD71670)
  
  

Field of view max in software 2048 x 2048 pixels

Environmental control:
Full chamber with heating control and Stage top incubation with CO₂ and humidity control (OKOLab)

Cameras:
sCMOS ORCA Fusion BT (Hamamatsu)

Computer:
HP Z4 High-End RTX A
Ecran incurve HP Z38c
Xeon W-2245 3.90GHz 3.91GHz 128Gb RAM

Acquisition Software:
NIS-Elements