LSM980 Airyscan2 8Y FLIM

User Manual

The Basic Principle of Airyscan

The Basic Principle of FLIM

The Basic Principle of FCS

Capabilities

• High sensitivity point scanning confocal imaging
• Spectral array GaAsP detectors
• AiryScan2 SR module with up to 1.5x resolution gain and high SNR
• AiryScan2 8Y module with up to 8x speed and high SNR
• Inverted microscope
• Incubation for live cell timelapse – temp and CO₂ control
• Timelapse with Definite focus2
• Multi-position
• Tiles acquisition and stitching of images
• BF and Nomarski contrast (DIC)
• FLIM and FCS module from Picoquant

Detailed Specifications

Zeiss Axio Observer Z.1 stand with Definite Focus2
Motorized XY stage
Piezo Z stage

Metal halide fluorescence source for widefield eyepiece visualization
with Blue, Green, Red fluorescence cubes

Full environmental chamber with heating, humidity and CO₂ control (Pecon)

Lasers:
405 nm, 445 nm, 488 nm, 561nm, 639nm Diode laser

Objectives:

• 10x/0.30 Plan-Apo WD: 5.2mm Air (420642-9800)
• 25x/0.8 Plan-Apochromat Imm LD LCI Corr DIC (420852-9870)
• 40x/1.3 Plan-Apochromat Oil DIC (420762-9800)
• 63x/1.4 Plan-Apochromat Oil DIC (420782-9900)

Detectors:

• GaAsP high QE 32 channel spectral array with unmixing capability
• 2 standard alkali PMTs in positions for blue and far-red detection
• AiryScan detector module and AiryScan Fast 4Y-8Y capability
• Transmitted light detector

Workstation Z6 (HP)
CPU: Intel Xeon ES-2623 v3 3.0 GHz; Memory: 32GB
Acquisition software Zen 3.5
Direct network link to Zeiss offline workstation – designed for Airyscan processing

FLIM and FCS module for LSM (PicoQuant) with Symphotime Software