Invité par l’équipe Prioleau, Ichiro Hiratani (RIKEN Center for Biosystems Dynamics Research (RIKEN BDR), Kobe, Japan) présentera un séminaire de l’Institut Jacques Monod sur le thème :
Unraveling the dynamic 3D genome architecture through single-cell DNA replication profiling
Résumé :
Hi-C technology has revolutionized genome biology, revealing that mammalian chromosomes are partitioned into megabase-sized topologically associating domains (TADs). TADs can be in either A (active) or B (inactive) nuclear compartments, which exhibit early and late DNA replication timing (RT), respectively. Several years ago, we developed a single-cell DNA replication sequencing technology, scRepli-seq, which has allowed us to monitor genome-wide RT dynamics and infer 3D genome organization dynamics in single cells. I will discuss our ongoing efforts to utilize scRepli-seq to understand the regulatory principles of 3D genome organization through the lens of DNA replication regulation. Topics include the 3D genome and replication dynamics during mammalian development, X-chromosome inactivation, and cell cycle, as well as our ongoing effort to understand the mechanism and significance of RT/compartment regulation.